The Automated Tangential-Flow Diafiltration Device presents an innovative and cost-effective solution for purifying and concentrating liquid suspensions of colloids, proteins, or cells. Traditional tangential flow filtration (TFF) devices are often expensive and complex, but this device leverages affordable materials and an Arduino microcontroller to create an accessible option for laboratory-scale applications. It is particularly designed for soft matter colloid research, enabling efficient buffer exchange and concentration processes.
Key Features
- Cost-Effective: The device costs approximately €2400, significantly less than typical lab-scale TFF systems that can range from €20,000 to €30,000.
- Automated Control: Integrated with an Arduino microcontroller for monitoring and controlling pressure and flow rates automatically during filtration processes.
- Utilizes Medical Membranes: Employs low-cost hemodialysis membrane modules that are significantly cheaper than standard TFF modules.
- Compact Design: Constructed to fit in standard lab environments without requiring extensive modifications.
- Wide Applicability: Suitable for various applications including colloidal purification, protein purification, and microbiological buffer exchange.
Specifications
| Specification | Details |
|---|---|
| Device Name | Automated Tangential-Flow Diafiltration Device |
| Cost | €2400 |
| Subject Areas | Chemical Engineering, Biochemistry, Polymer Chemistry |
| Hardware Type | Lab-scale chemical engineering unit operation system |
| Open Source License | CC BY-SA 4.0 |
| Source File Repository | Design Files |
Hardware Components
- Pumps: Uses diaphragm pumps to create the necessary transmembrane pressure.
- Membrane Module: Utilizes medical-grade hemodialysis membranes that are low-cost and effective for filtration tasks.
- Sensors: Includes pressure sensors and an ultrasonic sensor for level detection, ensuring precise control during operations.
Operation Instructions
- Setup: Install the membrane module, fill the sample beaker with the solution, and ensure the water tank is filled.
- Filtration: The sample solution flows tangentially across the membrane, allowing smaller molecules to pass while larger particles are retained.
- Diafiltration: The system automatically replaces lost volume with fresh buffer to maintain sample volume during the filtration process.
- Flushing: Conduct a flushing step to remove accumulated material from the membrane after filtration.
- Cleaning: After operation, clean the system with a suitable cleaning solution to maintain hygiene and functionality.
The Automated Tangential-Flow Diafiltration Device offers a revolutionary approach to filtration processes in polymer and biochemical research, dramatically lowering costs while maintaining high efficiency. Its automation and adaptability make it an ideal choice for researchers seeking to enhance their laboratory capabilities.
